RESUMO Introdução A correta compreensão e implementação das tarefas definidas pelo treinador para o jogador é alcançada através de uma ampla variedade de sessões de treinamento. Até hoje, a questão da eficácia do treinamento e a preparação dos jogadores profissionais de futebol para os jogos não foi suficientemente estudada. Objetivo Estudar os meios e métodos utilizados no treinamento de futebol e, por comparação, descobrir qual dos métodos é mais eficaz para obter um resultado positivo durante o jogo e manter a saúde dos jogadores. Métodos O estudo utilizou métodos matemáticos e físicos, assim como análise comparativa. No decorrer do estudo, foram considerados os principais métodos de treinamento em uma equipe. Foi realizada uma análise comparativa entre dois tipos de treinamento individual de jogadores de futebol, objetivando melhorar parâmetros físicos e técnicos. Resultado Foram estabelecidos quais parâmetros influenciam a escolha do esquema de treinamento. A eficácia de ambos os sistemas de treinamento é comprovada pelos indicadores estatísticos dos jogadores de futebol que treinam de acordo com estes métodos. Conclusão A eficácia da metodologia de treinamento escolhida por um jogador de futebol depende de suas habilidades físicas iniciais e habilidades profissionais. O significado prático do estudo é determinado pelo fato de que os métodos propostos podem ser utilizados no treinamento de atletas profissionais. Evidência nível II; Estudos terapêuticos – pesquisa de resultados.

ABSTRACT Introduction The correct understanding and implementation of the tasks set by the coach for the player are achieved through a wide variety of training sessions. To date, the question of training effectiveness and the preparation of professional soccer players for matches has not been sufficiently studied. Objective Study the means and methods used in soccer training. By comparison, find out which method is most effective in achieving a positive result during the game and maintaining the players’ health. Methods The study used mathematical and physical methods and comparative analysis. In the study, the main training methods in a team were considered. A comparative analysis was made between two types of individual soccer players’ training to improve physical and technical parameters. Result We established which parameters influence the choice of the training scheme. The effectiveness of both training systems is proven by the statistical indicators of soccer players who train according to these methods. Conclusion The effectiveness of the training methodology chosen by a soccer player depends on his initial physical abilities and professional skills. The study’s practical significance is determined by the fact that the proposed methods can be used in training professional athletes. Evidence level II; Therapeutic studies - outcomes research.

RESUMEN Introducción La correcta comprensión y ejecución de las tareas establecidas por el entrenador para el jugador se consigue a través de una amplia variedad de sesiones de entrenamiento. Hasta hoy, la cuestión de la eficacia del entrenamiento y la preparación de los futbolistas profesionales para los partidos no se ha estudiado suficientemente. Objetivo Estudiar los medios y métodos utilizados en el entrenamiento de fútbol y, por comparación, averiguar qué método es más eficaz para conseguir un resultado positivo durante el juego y mantener la salud de los jugadores. Métodos El estudio utilizó métodos matemáticos y físicos, así como análisis comparativos. En el transcurso del estudio, se consideraron los principales métodos de formación en un equipo. Se realizó un análisis comparativo entre dos tipos de entrenamiento individual de jugadores de fútbol, con el objetivo de mejorar los parámetros físicos y técnicos. Resultado Se estableció qué parámetros influyen en la elección del esquema de entrenamiento. La eficacia de ambos sistemas de entrenamiento queda demostrada por los indicadores estadísticos de los futbolistas que entrenan según estos métodos. Conclusión La eficacia de la metodología de entrenamiento elegida por un futbolista depende de sus capacidades físicas iniciales y de sus habilidades profesionales. La importancia práctica del estudio viene determinada por el hecho de que los métodos propuestos pueden utilizarse en el entrenamiento de atletas profesionales. Nivel de evidencia II; Estudios terapéuticos - investigación de resultados.

ABSTRACT Purpose: Interstitial cystitis/bladder pain syndrome (IC/BPS) is a devastating urological chronic pelvic pain condition. In search of a potential treatment, we investigated the effect of emodin on IC/BPS inflammation and fibrosis, and explore the potential mechanism. Methods: An experimental model of interstitial cystitis was induced by cyclophosphamide, and human bladder smooth muscle cells were treated with lipopolysaccharide to establish the cell model in vitro. In both models, inflammation- and fibrosis-related indexes were measured after emodin administration. Furthermore, the specific antagonists were used to dig for the mechanisms underlying the response to emodin treatment. Results: Emodin significantly ameliorated management of cystitis, reduced the amount of inflammatory cytokines (tumor necrosis factor-α, monocyte chemoattractant protein-1, interleukin-1β, interleukin-8, and interleukin-6) in models, as well as reducing the synthesis of fibrosis marker including collagen1, collagen3, vimentin, fibronectin and α-smooth muscle actin. Further mechanism studies demonstrated that emodin inhibited inflammatory reaction and fibrosis through blocking lysine-specific demethylase 6B (JMJD3) expression via JAK/STAT, NF-κB and TGF-β/SMAD pathways. Conclusions: Our study reveals the critical role of emodin-JMJD3 signaling in interstitial cystitis by regulating inflammation, fibrosis, and extracellular matrix deposition in cells and tissues, and these findings provide an avenue for effective treatment of patients with cystitis.

A comprehensive search of PubMed and Embase was performed in January 2015 to examine the available literature on validated diagnostic models of the pre-test probability of stable coronary artery disease and to describe the characteristics of the models. Studies that were designed to develop and validate diagnostic models of pre-test probability for stable coronary artery disease were included. Data regarding baseline patient characteristics, procedural characteristics, modeling methods, metrics of model performance, risk of bias, and clinical usefulness were extracted. Ten studies involving the development of 12 models and two studies focusing on external validation were identified. Seven models were validated internally, and seven models were validated externally. Discrimination varied between studies that were validated internally (C statistic 0.66-0.81) and externally (0.49-0.87). Only one study presented reclassification indices. The majority of better performing models included sex, age, symptoms, diabetes, smoking, and hyperlipidemia as variables. Only two diagnostic models evaluated the effects on clinical decision making processes or patient outcomes. Most diagnostic models of the pre-test probability of stable coronary artery disease have had modest success, and very few present data regarding the effects of these models on clinical decision making processes or patient outcomes.

ABSTRACT The effects of rheum on serum parameters in a taurocholate-induced acute pancreatitis (AP) rat model were investigated using pathological and biochemical tests, and a proton nuclear magnetic resonance (1H NMR)-based metabonomic strategy. Healthy rats and rats with AP were either treated with rheum (7.5% at a dose of 1.5 g/kg) or left untreated. Serum samples were collected from the AP and rheum-treated groups at 6, 12, and 24 h after treatment. The effect of rheum on pathological changes in the pancreatic was investigated to validate the AP model. We obtained 1H NMR spectra and analyzed the results using the partial least squares discriminant method. The results of the pathological and metabolic analyses revealed an amelioration of multiple metabolic abnormalities and an increase in the aerobic respiration ratio after treatment, compared with the AP groups. These results were attributed to improvements in energy supply and the elimination of metabolic products. The study also promoted NMR-based metabonomic analysis as a feasible method of assessing traditional Chinese drugs.

Plant pathogenic fungi have long been documented through concerted efforts of mycologists and plant pathologists; these records have served as the basis for regional and countrywide checklists which have since been put into databases listing hosts and associated fungi. They are used by governments and scientists to formulate trade quarantine policies and determine research funding, such as in plant breeding programs and disease control. With the ability to use molecular characters to study the systematics of fungi it is clear that morphologically defined species are often large complexes comprised of genetically and biologically distinct species. Use of molecular techniques to examine species complexes has revealed cryptic species in many important plant pathogenic genera, e.g. Botryosphaeria, Colletotrichum, Fusarium, and Mycosphaerella. It has occurred to such an extent that existing checklists and databases need updating. It is important that the data from these studies, including changes in taxonomy and nomenclature, be incorporated into the databases of plant pathogenic fungi to support accurate plant quarantine decisions. In addition, epitypifying fungi by re-collecting material from type habitats and isolating the organism into pure culture will provide essential materials for systematics studies to further clarify the taxonomy and phylogeny of plant pathogenic fungi. Overall, we conclude that disease lists are likely to be highly outdated and advocate the need for countrywide re-inventory of plant pathogens. As a result of these studies, tools can be developed that use morphological or molecular characters, or both, to promote accurate identification of plant pathogenic fungi.

The oncogene Bmi-1 is a member of the Polycomb group gene family. Its expression is found to be greatly increased in a number of malignant tumors including breast cancer. This could suggest Bmi-1 as a potent therapeutic target. In this study, RNAi was introduced to down-regulate the expression of Bmi-1 in a highly malignant breast adenocarcinoma cell line, MCF-7. A thorough study of the biological behavior and chemosensitivity changes of the MCF-7 cells was carried out in context to the therapeutic potential of Bmi-1. The results obtained indicated that siRNA targeting of Bmi-1 could lead to an efficient and specific inhibition of endogenous Bmi-1 activity. The mRNA and protein expression of Bmi-1 were determined by RT-PCR and Western blot, respectively. Furthermore, silencing of Bmi-1 resulted in a drastic inhibition of the growth of MCF-7 cells as well as G1/S phase transition. The number of target cells was found to increase in phase G0/G1 and decrease in the S phase, but no increase in the basal level of apoptosis was noticed. On the other hand, a reduction in the expression of cyclin D1 and an increase in the expression of p21 were also noticed. Silencing of Bmi-1 made the MCF-7 cells more sensitive to the chemotherapeutic agent doxorubicin and induced a significantly higher percentage of apoptotic cells. Here, we report on a study regarding the RNAi-mediated silencing of the Bmi-1 gene in breast cancer.